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nfκb p65 dna binding activity  (Active Motif)


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    Active Motif nfκb p65 dna binding activity
    Nfκb P65 Dna Binding Activity, supplied by Active Motif, used in various techniques. Bioz Stars score: 98/100, based on 1447 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nf%CE%BAb+p65+dna+binding+activity/TransAM+NF%CE%BAB+p65/pm41345286-424-13-27
    Average 98 stars, based on 1447 article reviews
    nfκb p65 dna binding activity - by Bioz Stars, 2026-11
    98/100 stars

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    Article Title: Defective vascular smooth muscle cell tafazzin impairs mitochondrial function and promotes atherosclerosis in preclinical models.
    Article Snippet: VSMCswere treatedwith 30 μmol/L tert-butyl hydrogen peroxide (Sigma) for 16 hours, 100μg/ml LDL (Sigma) for 24 h or 100μg/ml oxidised LDL for 24 h (Kalen Biomedical).

    Article Title: Defective vascular smooth muscle cell tafazzin impairs mitochondrial function and promotes atherosclerosis in preclinical models
    Article Snippet: For human microRNA 125a-5p experiments, human VSMCs were transfected with 25 nM mir-125a-5p mimic (C-300624-05-0005, Horizon Discovery), 50 nM mir-125a-5p inhibitor (IH-300624-06-0005, Horizon) or control ( CN-001000-01, Horizon).

    Activity Assay:

    Article Title: Significance of Tissue Expression and Serum Levels of Angiopoietin-like Protein 4 in Breast Cancer Progression: Link to NF-κB /P65 Activity and Pro-Inflammatory Cytokines.
    Article Snippet: .. 2-Determination of DNA-binding activity of NF-kB/p65 The ELISA-based TransAMTM NFκB p65 protein assay was used to evaluate NFκB/p65 DNA-binding activity (Cat # 40096, Active Motif, Carlsbad, CA, USA), according to the manufacturer’s instructions. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Significance of Tissue Expression and Serum Levels of Angiopoietin-like Protein 4 in Breast Cancer Progression: Link to NF-κB /P65 Activity and Pro-Inflammatory Cytokines.
    Article Snippet: .. 2-Determination of DNA-binding activity of NF-kB/p65 The ELISA-based TransAMTM NFκB p65 protein assay was used to evaluate NFκB/p65 DNA-binding activity (Cat # 40096, Active Motif, Carlsbad, CA, USA), according to the manufacturer’s instructions. ..



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    Figure 2. Resveratrol treatment suppressed the chronic unpredictable mild stress-induced changes of the protein levels of Sirt1, NF-κB <t>p65,</t> ac-p65, and Iκ-Bα in the mouse hippocampus via Western blot. *P < 0.05 vs. the control group; &P < 0.05 vs. the depression model group.
    Nf κb P65 Dna Binding Activity Assay Nf κb P65 Dna Binding Activity, supplied by Active Motif, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fig. 6. Molecular docking of Nicotiflorin with TLR4, <t>p65,</t> Caspase-1, NLRP3, and IL-1β. (A) Schematic diagram of molecular docking of Nicotiflorin with TLR4. (B) Schematic diagram of molecular docking of Nicotiflorin with p65. (C) Schematic diagram of molecular docking of Nicotiflorin with Caspase-1. (D) Schematic diagram of molecular docking of Nicotiflorin with NLRP3. (E) Schematic diagram of molecular docking of Nicotiflorin with IL-1β.
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    Fig. 6. Molecular docking of Nicotiflorin with TLR4, <t>p65,</t> Caspase-1, NLRP3, and IL-1β. (A) Schematic diagram of molecular docking of Nicotiflorin with TLR4. (B) Schematic diagram of molecular docking of Nicotiflorin with p65. (C) Schematic diagram of molecular docking of Nicotiflorin with Caspase-1. (D) Schematic diagram of molecular docking of Nicotiflorin with NLRP3. (E) Schematic diagram of molecular docking of Nicotiflorin with IL-1β.
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    Figure 2. Resveratrol treatment suppressed the chronic unpredictable mild stress-induced changes of the protein levels of Sirt1, NF-κB p65, ac-p65, and Iκ-Bα in the mouse hippocampus via Western blot. *P < 0.05 vs. the control group; &P < 0.05 vs. the depression model group.

    Journal: Future science OA

    Article Title: Resveratrol alleviates depressive-like behavior via the activation of SIRT1/NF-κB signaling pathway in microglia.

    doi: 10.1080/20565623.2025.2463852

    Figure Lengend Snippet: Figure 2. Resveratrol treatment suppressed the chronic unpredictable mild stress-induced changes of the protein levels of Sirt1, NF-κB p65, ac-p65, and Iκ-Bα in the mouse hippocampus via Western blot. *P < 0.05 vs. the control group; &P < 0.05 vs. the depression model group.

    Article Snippet: NF-κB p65 DNA binding activity assay nF-κB p65 dna binding activity was detected using transam nF-κB p65 Chemi tran-scription Factor assay Kit (active motif, Carlsbad, Ca) according to the instructions of manufacturer.

    Techniques: Western Blot, Control

    Figure 3. Resveratrol treatment suppressed the chronic unpredictable mild stress-induced changes of the protein levels of Sirt1, NF-κB p65, ac-p65, and Iκ-Bα in the mouse prefrontal cortex via Western blot. *P < 0.05 vs. the control group; &P < 0.05 vs. the depression model group.

    Journal: Future science OA

    Article Title: Resveratrol alleviates depressive-like behavior via the activation of SIRT1/NF-κB signaling pathway in microglia.

    doi: 10.1080/20565623.2025.2463852

    Figure Lengend Snippet: Figure 3. Resveratrol treatment suppressed the chronic unpredictable mild stress-induced changes of the protein levels of Sirt1, NF-κB p65, ac-p65, and Iκ-Bα in the mouse prefrontal cortex via Western blot. *P < 0.05 vs. the control group; &P < 0.05 vs. the depression model group.

    Article Snippet: NF-κB p65 DNA binding activity assay nF-κB p65 dna binding activity was detected using transam nF-κB p65 Chemi tran-scription Factor assay Kit (active motif, Carlsbad, Ca) according to the instructions of manufacturer.

    Techniques: Western Blot, Control

    Figure 5. Resveratrol treatment suppressed the LPS-induced changes of the protein levels of Sirt1, NF-κB p65, ac-p65, and Iκ-Bα in BV2 cells via Western blot. *P < 0.05 vs. the control group; **P < 0.01 vs. the control group; ***P < 0.001 vs. the control group.

    Journal: Future science OA

    Article Title: Resveratrol alleviates depressive-like behavior via the activation of SIRT1/NF-κB signaling pathway in microglia.

    doi: 10.1080/20565623.2025.2463852

    Figure Lengend Snippet: Figure 5. Resveratrol treatment suppressed the LPS-induced changes of the protein levels of Sirt1, NF-κB p65, ac-p65, and Iκ-Bα in BV2 cells via Western blot. *P < 0.05 vs. the control group; **P < 0.01 vs. the control group; ***P < 0.001 vs. the control group.

    Article Snippet: NF-κB p65 DNA binding activity assay nF-κB p65 dna binding activity was detected using transam nF-κB p65 Chemi tran-scription Factor assay Kit (active motif, Carlsbad, Ca) according to the instructions of manufacturer.

    Techniques: Western Blot, Control

    Fig. 6. Molecular docking of Nicotiflorin with TLR4, p65, Caspase-1, NLRP3, and IL-1β. (A) Schematic diagram of molecular docking of Nicotiflorin with TLR4. (B) Schematic diagram of molecular docking of Nicotiflorin with p65. (C) Schematic diagram of molecular docking of Nicotiflorin with Caspase-1. (D) Schematic diagram of molecular docking of Nicotiflorin with NLRP3. (E) Schematic diagram of molecular docking of Nicotiflorin with IL-1β.

    Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

    Article Title: Mechanism of Nicotiflorin in San-Ye-Qing rhizome for anti-inflammatory effect in ulcerative colitis.

    doi: 10.1016/j.phymed.2024.155564

    Figure Lengend Snippet: Fig. 6. Molecular docking of Nicotiflorin with TLR4, p65, Caspase-1, NLRP3, and IL-1β. (A) Schematic diagram of molecular docking of Nicotiflorin with TLR4. (B) Schematic diagram of molecular docking of Nicotiflorin with p65. (C) Schematic diagram of molecular docking of Nicotiflorin with Caspase-1. (D) Schematic diagram of molecular docking of Nicotiflorin with NLRP3. (E) Schematic diagram of molecular docking of Nicotiflorin with IL-1β.

    Article Snippet: NF-κB p65 DNA binding activity was measured with the TransAM NF-κB p65 Activation Assay kit (40096, Active Motif, USA) following the manufacturer’s protocol.

    Techniques:

    Fig. 7. p65 is a Key Target of Nicotiflorin. (A) Flowchart of the CETSA assay. (B) WB validation results of CETSA assay samples. The p65 protein treated with nicotiflorin had better thermal stability. (C) Analysis of relative grey values of WB validation results of CETSA assay samples. (D) Flowchart of the DARTS assay.(E) WB validation results of DARTS assay samples. The p65 protein treated with Nicotiflorin was more tolerant to pronase. (F-G) Biacore analysis results of Nicotiflorin and p65. The dots represents various concentrations of Nicotiflorin. Nicotiflorin binds to p65 with a KD of 47.39 nM. (H) The enzyme activity of p65. The activity of p65 protein in the protein treated with Nicotiflorin decreased significantly.

    Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

    Article Title: Mechanism of Nicotiflorin in San-Ye-Qing rhizome for anti-inflammatory effect in ulcerative colitis.

    doi: 10.1016/j.phymed.2024.155564

    Figure Lengend Snippet: Fig. 7. p65 is a Key Target of Nicotiflorin. (A) Flowchart of the CETSA assay. (B) WB validation results of CETSA assay samples. The p65 protein treated with nicotiflorin had better thermal stability. (C) Analysis of relative grey values of WB validation results of CETSA assay samples. (D) Flowchart of the DARTS assay.(E) WB validation results of DARTS assay samples. The p65 protein treated with Nicotiflorin was more tolerant to pronase. (F-G) Biacore analysis results of Nicotiflorin and p65. The dots represents various concentrations of Nicotiflorin. Nicotiflorin binds to p65 with a KD of 47.39 nM. (H) The enzyme activity of p65. The activity of p65 protein in the protein treated with Nicotiflorin decreased significantly.

    Article Snippet: NF-κB p65 DNA binding activity was measured with the TransAM NF-κB p65 Activation Assay kit (40096, Active Motif, USA) following the manufacturer’s protocol.

    Techniques: Biomarker Discovery, Activity Assay

    Fig. 8. Nicotiflorin suppresses inflammation in vitro by inhibiting NF-κB activation and NLRP3 inflammasome activation. (A) Expression of p-p65, p65, p- IκBα, and IκBα of NF-κB signaling pathway species in cells after addition of Nicotiflorin to BMDMs. (B-D) Concentrations of IL-1β, IL-6, and IL-18 in cell supernatants after addition of Nicotiflorin to BMDMs. (E) Expression of Caspase-1, IL-1β, and NLRP3 in cells and expression of Caspase-1 p20, IL-1β p17 in cell supernatants after addition of Nicotiflorin to BMDMs (ns p ≥0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

    Article Title: Mechanism of Nicotiflorin in San-Ye-Qing rhizome for anti-inflammatory effect in ulcerative colitis.

    doi: 10.1016/j.phymed.2024.155564

    Figure Lengend Snippet: Fig. 8. Nicotiflorin suppresses inflammation in vitro by inhibiting NF-κB activation and NLRP3 inflammasome activation. (A) Expression of p-p65, p65, p- IκBα, and IκBα of NF-κB signaling pathway species in cells after addition of Nicotiflorin to BMDMs. (B-D) Concentrations of IL-1β, IL-6, and IL-18 in cell supernatants after addition of Nicotiflorin to BMDMs. (E) Expression of Caspase-1, IL-1β, and NLRP3 in cells and expression of Caspase-1 p20, IL-1β p17 in cell supernatants after addition of Nicotiflorin to BMDMs (ns p ≥0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Article Snippet: NF-κB p65 DNA binding activity was measured with the TransAM NF-κB p65 Activation Assay kit (40096, Active Motif, USA) following the manufacturer’s protocol.

    Techniques: In Vitro, Activation Assay, Expressing